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Multimodal vascular imaging (e.g., combined MRI and PET, ultrasound, and CT) can combine the strengths of different imaging modalities and provide new insights into vascular aging mechanisms

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For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent